Implantation of engineered human microvasculature to study human infectious diseases in mouse models

iScience. 2023 Feb 27;26(4):106286. doi: 10.1016/j.isci.2023.106286. eCollection 2023 Apr 21.


Animal models for studying human pathogens are crucially lacking. We describe the implantation in mice of engineered human mature microvasculature consisting of endothelial and perivascular cells embedded in collagen hydrogel that allows investigation of pathogen interactions with the endothelium, including in vivo functional studies. Using Neisseria meningitidis as a paradigm of human-restricted infection, we demonstrated the strength and opportunities associated with the use of this approach.

Keywords: Bioengineering; Biological sciences; Cell biology; Microbiology.