Microsequencing of dopamine beta-monooxygenase

J Neurosci Res. 1987;18(2):289-92. doi: 10.1002/jnr.490180204.

Abstract

Tryptic peptides and cyanogen bromide fragments of dopamine beta-monooxygenase (DBH) were prepared and separated on C-8 reverse phase columns by high pressure liquid chromatography. Absorbance profiles at both 220 nm and 280 nm were monitored so that peptides with aromatic residues could be isolated. These peptides were subjected to automated Edman degradation with a gas phase microsequencer.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Chromatography, High Pressure Liquid
  • Dopamine beta-Hydroxylase / analysis*
  • Molecular Sequence Data
  • Peptide Fragments
  • Peptide Mapping

Substances

  • Peptide Fragments
  • Dopamine beta-Hydroxylase