Process characteristics of cell lysis mutants of Saccharomyces cerevisiae

Biotechnol Bioeng. 1979 Apr;21(4):659-70. doi: 10.1002/bit.260210410.


Several temperature-sensitive lysis mutants of Saccharomyces cerevisiae were selected according to their ability to release alkaline phosphatase when incubated at a nonpermissive temperature. For two mutants, cell lysis and release of alkaline phosphatase reached a maximum when cells in the logarithmic growth phase were shifted to the nonpermissive temperature. Morphological changes, as well as changes in macromolecular composition of the cells, were observed. Growth is necessary and oxygen is important for the expression of cell lysis at the nonpermissive temperature.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Alkaline Phosphatase / metabolism*
  • Cell Division
  • Ethanol / pharmacology
  • Fungal Proteins / metabolism*
  • Mutation
  • RNA / metabolism
  • Saccharomyces cerevisiae / cytology*
  • Saccharomyces cerevisiae / drug effects
  • Saccharomyces cerevisiae / enzymology
  • Saccharomyces cerevisiae / metabolism
  • Temperature


  • Fungal Proteins
  • Ethanol
  • RNA
  • Alkaline Phosphatase