Generation of a PPP1CA knockout human pluripotent stem cell line via CRISPR/Cas9

Stem Cell Res. 2023 Jun:69:103077. doi: 10.1016/j.scr.2023.103077. Epub 2023 Mar 21.


The Protein phosphatase 1 catalytic subunit alpha (PPP1CA) is a alpha subunit of the PP1 complex, which known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Increased PP1 activity has been observed in the end stage of heart failure. Here, a PPP1CA knockout human embryonic stem cell line, WAe009-A-B, was generated using the CRISPR/Cas9 system to further study the function of PPP1CA. The cell line confirmed with pluripotency, normal karyotype and differentiation potential.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • CRISPR-Cas Systems* / genetics
  • Cell Line
  • Embryonic Stem Cells / metabolism
  • Human Embryonic Stem Cells* / metabolism
  • Humans
  • Protein Phosphatase 1 / genetics
  • Protein Phosphatase 1 / metabolism


  • Protein Phosphatase 1
  • PPP1CA protein, human