Nucleotide sequence of the regulatory locus controlling expression of bacterial genes for bioluminescence

Nucleic Acids Res. 1987 Dec 23;15(24):10455-67. doi: 10.1093/nar/15.24.10455.


Production of light by the marine bacterium Vibrio fischeri and by recombinant hosts containing cloned lux genes is controlled by the density of the culture. Density-dependent regulation of lux gene expression has been shown to require a locus consisting of the luxR and luxI genes and two closely linked divergent promoters. As part of a genetic analysis to understand the regulation of bioluminescence, we have sequenced the region of DNA containing this control circuit. Open reading frames corresponding to luxR and luxI were identified; transcription start sites were defined by S1 nuclease mapping and sequences resembling promoter elements were located.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Bacterial Proteins / genetics*
  • Base Sequence
  • Cloning, Molecular
  • DNA, Bacterial / genetics
  • Gene Expression Regulation
  • Genes, Bacterial*
  • Genes, Regulator*
  • Luminescent Measurements*
  • Molecular Sequence Data
  • Operon*
  • Transcription, Genetic
  • Vibrio / genetics*


  • Bacterial Proteins
  • DNA, Bacterial