Substrate Specificity of DNA Polymerases. II. 5-(1-Alkynyl)-dUTPs as Substrates of the Klenow DNA Polymerase Enzyme

Nucleic Acids Symp Ser. 1987;(18):125-9.

Abstract

A structure-activity relationship study was carried out with a sequence of 5-(1-alkynyl)-dUTPs in Klenow DNA polymerase enzyme-catalyzed reactions. Ability of modified dUTPs examined (up to the octynyl derivative) to serve as a substrate depended on both the length of substituent and the structure of DNA primer-template used.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA-Directed DNA Polymerase / metabolism*
  • Deoxyuracil Nucleotides* / chemical synthesis*
  • Kinetics
  • Substrate Specificity

Substances

  • Deoxyuracil Nucleotides
  • deoxyuridine triphosphate
  • DNA-Directed DNA Polymerase