Adenovirus VAI RNA antagonizes the antiviral action of interferon by preventing activation of the interferon-induced eIF-2 alpha kinase

Cell. 1986 Apr 25;45(2):195-200. doi: 10.1016/0092-8674(86)90383-1.


The VAI RNA of adenovirus is a small, RNA polymerase III-transcribed species required for efficient translation of host cell and viral mRNAs late after infection. The growth of a viral mutant that is unable to produce the RNA is inhibited by interferon, while wild-type virus is not affected. VAI RNA prevents activation of the interferon-induced P1/eIF-2 alpha kinase. This inhibition can be reproduced in extracts of interferon-treated cells where purified VAI RNA prevents activation of latent kinase by double-stranded RNA.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adenoviruses, Human / drug effects
  • Adenoviruses, Human / genetics
  • Adenoviruses, Human / physiology*
  • Cell Line
  • Embryo, Mammalian
  • Enzyme Activation / drug effects
  • Humans
  • Interferon Type I / pharmacology*
  • Kidney
  • Phosphorylation
  • Protein Biosynthesis
  • Protein Kinase Inhibitors*
  • RNA, Double-Stranded / pharmacology
  • RNA, Small Nuclear / physiology*
  • RNA, Viral / physiology*
  • eIF-2 Kinase


  • Interferon Type I
  • Protein Kinase Inhibitors
  • RNA, Double-Stranded
  • RNA, Small Nuclear
  • RNA, Viral
  • eIF-2 Kinase