Liquid chromatography used in diagnosis of a rare hemoglobin combination: hemoglobin S/LeporeBoston

Clin Chem. 1986 May;32(5):903-6.


"High-performance" liquid chromatography (HPLC), applied to hemoglobin analysis, is decidely more sensitive and gives better resolution than do routine electrophoretic methods. Here we present a case with a rare double heterozygote hemoglobin S/LeporeBoston, originally diagnosed as homozygous hemoglobin S by routine electrophoretic methods. Using a gradient elution weak cation-exchange HPLC technique, we could separate hemoglobin S and hemoglobin LeporeBoston and make the correct diagnosis. This case demonstrates how HPLC can be a useful adjunct to routine electrophoresis.

Publication types

  • Case Reports
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Anemia, Sickle Cell / blood
  • Anemia, Sickle Cell / genetics
  • Child
  • Chromatography, High Pressure Liquid / methods
  • Electrophoresis, Cellulose Acetate
  • Female
  • Hemoglobin, Sickle / analysis*
  • Hemoglobins, Abnormal / analysis*
  • Humans
  • Male


  • Hemoglobin, Sickle
  • Hemoglobins, Abnormal
  • hemoglobin Lepore Boston