A sensitive and specific enzyme-linked immunosorbent assay for the detection of pymetrozine in vegetable, cereal, and meat

Xiaoyang Yin; Huaming Li; Shuangmin Wu; Yanrong Lu; Yalin Yang; Liangni Qin; Long Li; Jiaxu Xiao; Jixiang Liang; Yu Si; Tao Le; Dapeng Peng

doi:10.1016/j.foodchem.2023.135949

A sensitive and specific enzyme-linked immunosorbent assay for the detection of pymetrozine in vegetable, cereal, and meat

Food Chem. 2023 Aug 30:418:135949. doi: 10.1016/j.foodchem.2023.135949. Epub 2023 Mar 16.

Authors

Xiaoyang Yin¹, Huaming Li¹, Shuangmin Wu¹, Yanrong Lu¹, Yalin Yang¹, Liangni Qin¹, Long Li¹, Jiaxu Xiao¹, Jixiang Liang¹, Yu Si¹, Tao Le², Dapeng Peng³

Affiliations

¹ National Reference Laboratory of Veterinary Drug Residues (HZAU) and MOA Key Laboratory for Detection of Veterinary Drug Residues, Huazhong Agricultural University, Wuhan 430070, PR China.
² College of Life Sciences, Chongqing Normal University, Chongqing 401331, PR China. Electronic address: letao@cqnu.edu.cn.
³ National Reference Laboratory of Veterinary Drug Residues (HZAU) and MOA Key Laboratory for Detection of Veterinary Drug Residues, Huazhong Agricultural University, Wuhan 430070, PR China; State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, PR China; Shenzhen Institute of Nutrition and Health, Huazhong Agricultural University, Wuhan 430070, PR China; Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518000, PR China. Electronic address: pengdapeng@mail.hzau.edu.cn.

PMID: 36989652
DOI: 10.1016/j.foodchem.2023.135949

Abstract

Pymetrozine is a neonicotinoid insecticide with high efficacy against aphids and planthoppers, and has been used worldwide. To monitor its residue in food, a highly specific and sensitive monoclonal antibody (McAb) was prepared, and an indirect competitive enzyme-linked immunosorbent assay (icELISA) was developed to detect pymetrozine, with a 50% inhibition value (IC₅₀) of 7.70 μg/L. The McAb showed little affinity for acetamiprid, hexazinone, metamitron, nitenpyram, metribuzin, and imidacloprid. The limits of detection (LOD) calculated from the analysis of broccoli, cabbage, wheat, maize, rice, chicken, fish, and crayfish samples were from 1.56 to 2.72 μg/kg and the average recoveries were from 81.25 to 103.19%. icELISA was confirmed using liquid chromatography-tandem mass spectrometry (LC-MS/MS). These results demonstrated that the optimised icELISA is a convenient and effective analytical tool for monitoring pymetrozine residues in food.

Keywords: Food; Indirect competitive enzyme-linked immunosorbent assay; Monoclonal antibody; Pymetrozine.

MeSH terms

Animals
Antibodies, Monoclonal
Brassica*
Chromatography, Liquid
Edible Grain / chemistry
Enzyme-Linked Immunosorbent Assay / methods
Meat / analysis
Tandem Mass Spectrometry
Vegetables* / chemistry

Substances

pymetrozine
Antibodies, Monoclonal