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Comparative Study
, 200 (1), 91-6

A Step Sensitive to Pertussis Toxin and Phorbol Ester in Human Neutrophils Regulates Chemotaxis and Capping but Not Phagocytosis

Comparative Study

A Step Sensitive to Pertussis Toxin and Phorbol Ester in Human Neutrophils Regulates Chemotaxis and Capping but Not Phagocytosis

P M Lad et al. FEBS Lett.

Abstract

Treatment of human neutrophils with pertussis toxin (PT) abolishes chemotaxis in response to either platelet-activating factor (PAF) or f-Met-Leu-Phe (FMLP), and capping induced via the concanavalin A (Con A) receptor. These functional effects are accompanied by the inhibition of calcium mobilization by PAF, FMLP and Con A. The agent phorbol 12-myristate-13-acetate (PMA) also inhibits chemotaxis and capping as well as calcium mobilization by these receptors. In sharp contrast, neither PT, cholera toxin (CT), nor PMA, inhibits the phagocytosis of non-opsonized and opsonized Candida albicans, sheep erythrocytes or fluorescent latex beads. Our results suggest that receptor-initiated chemotaxis and capping involve a step that is sensitive to PT and PMA, and that phagocytosis is not regulated in a similar fashion.

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