Depth and contaminant-shaped bacterial community structure and assembly at an aged chlorinated aliphatic hydrocarbon-contaminated site

Abstract

Chlorinated aliphatic hydrocarbons (CAHs) are potentially toxic substances that have been detected in various contaminated environments. Biological elimination is the main technique of detoxifying CAHs in the contaminated sites, but the soil bacterial community at CAH-contaminated sites have been little investigated. Here, high-throughput sequencing analysis of soil samples from different depths (to 6 m depth) at an aged CAH-contaminated site has been conducted to investigate the community composition, function, and assembly of soil bacteria. The alpha diversity of the bacterial community significantly increased with increasing depth and bacterial community also became more convergent with increasing depth. Organohalide-respiring bacteria (OHRB) is considered keystone taxa to reduce the environmental stress of CAHs by reductive dechlorinate CAHs into nontoxic products, increases the alpha diversity of bacterial community and improves the stability of bacterial co-occurrence network. The high concentration of CAHs in deep soil and the stable anaerobic environment make deterministic processes dominate bacterial community assembly, while the topsoil is dominated by dispersal limitation. In general, CAHs at contaminated sites have a great impact on bacterial community, but the CAHs metabolic community acclimated in deep soil can reduce the environmental stress of CAHs, which provides foundation for the monitored natural attenuation technology in CAHs-contaminated sites.

Keywords: Aged CAH-contaminated site; Bacterial community; Co-occurrence network; Community assembly; Soil depth.