In recent years, lipidomics have been widely developed to try to better understand many diseases or physical conditions. In this study, the aim was to evaluate the possibility to conduct reliable lipidomic studies using hemaPEN® microsampling devices. Targeted lipidomic analysis was applied to investigate the impact of a short and intense physical activity on lipids blood concentration. HemaPEN® microsampling device was used to easily collect several samples directly on an athletics track. This device allows the accurate collection of four blood samples (2.74 µL each) in a non-invasive way and without any specific skills. In this study, nineteen healthy volunteers aged from 19 to 27 were included. Participants ran 400 m warm-up and 1600 m as fast as possible. Blood samples were collected at five different time points. One sample was collected before the exercise, two during the physical activity and two after. An extraction process as well as an ultra-high-performance liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS) method were optimized to follow-up 11 compounds in these small volumes of blood. Blood concentration of five out of the eleven targeted analytes were significantly influenced by the physical exercise. Blood concentration of arachidonic acid, sphingosine and lactic acid were significantly increased after exercise, while concentration of 14:0 lysophosphatidylcholine and 18:1 lysophosphatidylcholine were significantly decreased.
Keywords: HemaPEN®; Microsampling; Physical activity; Targeted lipidomics; UHPLC-MS/MS.
Copyright © 2023 Elsevier B.V. All rights reserved.