Nanoencapsulation Motivates the High Inhibitive Ability of Fucoxanthin on H2O2-Induced Human Hepatocyte Cell Line (L02) Apoptosis via Regulating Lipid Metabolism Homeostasis

Chunyan Wang; Ying Bai; Wei Yin; Bixiang Qiu; Pengfei Jiang; Xiuping Dong; Hang Qi

doi:10.1021/acs.jafc.3c01160

Nanoencapsulation Motivates the High Inhibitive Ability of Fucoxanthin on H₂O₂-Induced Human Hepatocyte Cell Line (L02) Apoptosis via Regulating Lipid Metabolism Homeostasis

J Agric Food Chem. 2023 Apr 7. doi: 10.1021/acs.jafc.3c01160. Online ahead of print.

Authors

Chunyan Wang¹, Ying Bai¹, Wei Yin², Bixiang Qiu³, Pengfei Jiang¹, Xiuping Dong¹, Hang Qi¹

Affiliations

¹ School of Food Science and Technology, Dalian Polytechnic University, National Engineering Research Center of Seafood, Dalian 116034, People's Republic of China.
² Dalian Gaishi Food Co., Ltd., Dalian 116047, People's Republic of China.
³ Fujian Yida Food Co., Ltd., Fuzhou 350500, People's Republic of China.

PMID: 37026562
DOI: 10.1021/acs.jafc.3c01160

Abstract

This study reports an encapsulation system for fucoxanthin (FX) through simple affinity binding with gelatin (GE) and then coating with chitosan oligosaccharides (COS). The effects of FX before and after encapsulation on the human hepatocyte cell line (L02) were investigated. FX-GE and FX-GE-COS nanocomplexes exhibited a spherical shape with diameters of 209 ± 6 to 210 ± 8 nm. FX-GE-COS nanocomplexes were found to perform the best with the highest encapsulation efficiency (EE, 83.88 ± 4.39%), improved FX stability, and enhanced cellular uptake on the nanoscale. The cytotoxicity and cell mitochondrial damage of H₂O₂ exposure to L02 cells decreased with the increase of free-FX and FX-GE-COS nanocomplexes. FX-GE-COS nanocomplexes' intervention decreased the intracellular ROS and inhibited the apoptosis of L02 cells that was induced by H₂O₂ exposure in a concentration-dependent manner. Lipidomic analysis revealed that FX-GE-COS nanocomplexes could regulate the lipid metabolism disturbed by H₂O₂ and protected the mitochondrial function of L02 cells. These results suggested that nanoencapsulation enhanced the antioxidant activity of FX to L02 cells, and the constructed FX-GE-COS nanocomplexes have the potential to be an antioxidant nutritional dietary supplement.

Keywords: Antioxidant; Chitosan oligosaccharides; Encapsulation stability; Fucoxanthin; Gelatin; Lipid metabolism.