Report of genome sequence of Rhodococcus biphenylivorans strain GA1, an isolate capable of efficiently degrading lignin and its derivates

Haiyan Gao; Yifan Feng; Yujia Jiang; Wenming Zhang; Wankui Jiang; Fengxue Xin; Minjiao Chen; Min Jiang

doi:10.1007/s13205-023-03544-9

Report of genome sequence of Rhodococcus biphenylivorans strain GA1, an isolate capable of efficiently degrading lignin and its derivates

3 Biotech. 2023 May;13(5):124. doi: 10.1007/s13205-023-03544-9. Epub 2023 Apr 7.

Authors

Haiyan Gao¹, Yifan Feng¹, Yujia Jiang¹, Wenming Zhang^{1

2}, Wankui Jiang¹, Fengxue Xin^{1

2}, Minjiao Chen¹, Min Jiang^{1

2}

Affiliations

¹ State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Puzhu South Road 30#, Nanjing, 211800 People's Republic of China.
² Jiangsu Ational Synergetic Innovation Center for Advanced Materials (SICAM), Nanjing Tech University, Nanjing, 211800 People's Republic of China.

Abstract

Rhodococcus biphenylivorans GA1 was successfully isolated, which can efficiently degrade alkali lignin and a variety of lignin-derived aromatic compounds as the sole carbon source. Whole genome sequencing of strain GA1 showed that it possessed G + C content of 68% with the size of 6.0 Mb and 4319 putative open reading frames (ORFs). Four replicons consisting of one circular chromosome (ChrA1) and three circular plasmids (pGA1, pGA2, pGA3) were found. Among these annotated proteins, lignin depolymerizing peroxidases (Dyp) and two lignin-derived aromatic compounds cleavage dioxygenases, protocatechuate 3,4-dioxygenase(P34D) and catechol-1,2-dioxygenase (C12D) play key roles in the catabolism of lignin.

Keywords: Catabolism; Dioxygenase; Genome sequencing; Lignin; Peroxidases; Rhodococcus.

Publication types

Case Reports