Somatic Cell Nuclear Transfer in Pigs

Werner G Glanzner; Vitor B Rissi; Vilceu Bordignon

doi:10.1007/978-1-0716-3064-8_10

Somatic Cell Nuclear Transfer in Pigs

Methods Mol Biol. 2023:2647:197-210. doi: 10.1007/978-1-0716-3064-8_10.

Authors

Werner G Glanzner¹, Vitor B Rissi², Vilceu Bordignon³

Affiliations

¹ Department of Animal Science, McGill University, Sainte-Anne-de-Bellevue, QC, Canada.
² Faculty of Veterinary Medicine, Federal University of Santa Catarina, UFSC, Curitibanos, SC, Brazil.
³ Department of Animal Science, McGill University, Sainte-Anne-de-Bellevue, QC, Canada. vilceu.bordignon@mcgill.ca.

PMID: 37041336
DOI: 10.1007/978-1-0716-3064-8_10

Abstract

Somatic cell nuclear transfer (SCNT) has been successfully applied to clone animals of several species. Pigs are one of the main livestock species for food production and are also important for biomedical research due to their physiopathological similarities with humans. In the past 20 years, clones of several swine breeds have been produced for a variety of purposes, including biomedical and agricultural applications. In this chapter, we describe a protocol to produce cloned pigs by SCNT.

Keywords: Embryo transfer; Enucleation; Histone deacetylases; Nuclear reprogramming; Nuclear transplantation; SCNT; Sus scrofa; Swine; Transcriptional inhibition.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cloning, Organism* / methods
Humans
Nuclear Transfer Techniques*
Swine