MRC-5 Human Lung Fibroblasts Alleviate the Genotoxic Effect of Fe-N Co-Doped Titanium Dioxide Nanoparticles through an OGG1/2-Dependent Reparatory Mechanism

Int J Mol Sci. 2023 Mar 29;24(7):6401. doi: 10.3390/ijms24076401.

Abstract

The current study was focused on the potential of pure P25 TiO2 nanoparticles (NPs) and Fe(1%)-N co-doped P25 TiO2 NPs to induce cyto- and genotoxic effects in MRC-5 human pulmonary fibroblasts. The oxidative lesions of P25 NPs were reflected in the amount of 8-hydroxydeoxyguanosine accumulated in DNA and the lysosomal damage produced, but iron-doping partially suppressed these effects. However, neither P25 nor Fe(1%)-N co-doped P25 NPs had such a serious effect of inducing DNA fragmentation or activating apoptosis signaling. Moreover, oxo-guanine glycosylase 1/2, a key enzyme of the base excision repair mechanism, was overexpressed in response to the oxidative DNA deterioration induced by P25 and P25-Fe(1%)-N NPs.

Keywords: MRC-5 cells; OGG1/2 DNA repair glycosylase; P25 Degussa nanoparticles; base excision repair; human pulmonary fibroblasts; iron doping; nanoparticle genotoxicity; titanium dioxide.

MeSH terms

  • DNA / pharmacology
  • DNA Damage
  • Fibroblasts
  • Humans
  • Lung
  • Metal Nanoparticles* / toxicity
  • Nanoparticles*
  • Titanium / toxicity

Substances

  • titanium dioxide
  • Titanium
  • DNA