Effects of copper sulfate algaecide on the cell growth, physiological characteristics, the metabolic activity of Microcystis aeruginosa and raw water application

Hanyan Liu; Shengnan Chen; Haihan Zhang; Na Wang; Ben Ma; Xiang Liu; Limin Niu; Fan Yang; Yue Xu; Xiaoli Zhang

doi:10.1016/j.jhazmat.2022.130604

Effects of copper sulfate algaecide on the cell growth, physiological characteristics, the metabolic activity of Microcystis aeruginosa and raw water application

J Hazard Mater. 2023 Mar 5:445:130604. doi: 10.1016/j.jhazmat.2022.130604. Epub 2022 Dec 15.

Authors

Hanyan Liu¹, Shengnan Chen², Haihan Zhang¹, Na Wang¹, Ben Ma¹, Xiang Liu¹, Limin Niu¹, Fan Yang¹, Yue Xu¹, Xiaoli Zhang¹

Affiliations

¹ Shaanxi Key Laboratory of Environmental Engineering, Key Laboratory of Northwest Water Resource, Environment and Ecology, MOE, Xi'an University of Architecture and Technology, Xi'an 710055, China; School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China.
² Shaanxi Key Laboratory of Environmental Engineering, Key Laboratory of Northwest Water Resource, Environment and Ecology, MOE, Xi'an University of Architecture and Technology, Xi'an 710055, China; School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China. Electronic address: chenshengnan@xauat.edu.cn.

PMID: 37056015
DOI: 10.1016/j.jhazmat.2022.130604

Abstract

Harmful cyanobacteria blooms (HCBs) occurred frequently and become a serious scientific challenge. Copper sulfate (CuSO₄) is a broad-spectrum chemical algaecide to control algae blooms. Herein, the Microcystis aeruginosa was exposed to different CuSO₄ (0.0, 0.2 and 0.5 mg/L) to assess the variations in algal physiological process and metabolic profiles. The results indicated that exposure to CuSO₄ of 0.5 mg/L at 72 h could significantly inhibit the cell growth and photosynthetic capacity of M. aeruginosa, including chl-a content and chlorophyll fluorescence parameters. Plasma membrane damage causing cell lysis of M. aeruginosa increased the K⁺ release. The increase of SOD and CAT suggested that CuSO₄ treatment caused oxidative stress in algal cells. Different doses of CuSO₄ modified the carbon metabolic potential, algal cells had their unique metabolic mode thereby. Moreover, the research further verified that CuSO₄ would also inhibit algal growth and change algal community structure in site-collected water application. Overall, laboratory results of M. aeruginosa to CuSO₄ and site-collected water application of algal responses to CuSO₄ might be conducive to uncovering the controlling mechanism of algae and the potential effect of carbon cycling in an ecological environment.

Keywords: Biolog ECO-Plates; Community structure; Copper sulfate; Harmful algal blooms; Microcystis aeruginosa; Oxidation stress.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Carbon / metabolism
Copper Sulfate / toxicity
Herbicides* / metabolism
Microcystis*
Water / pharmacology

Substances

Copper Sulfate
Herbicides
Water
Carbon