Evaluation of post-natal angiogenesis in a mouse hind limb ischemia model

Cristina Arce Recatalá; Mattia Albiero; Massimo Mattia Santoro

doi:10.1016/j.xpro.2023.102232

Evaluation of post-natal angiogenesis in a mouse hind limb ischemia model

STAR Protoc. 2023 Apr 17;4(2):102232. doi: 10.1016/j.xpro.2023.102232. Online ahead of print.

Authors

Cristina Arce Recatalá¹, Mattia Albiero², Massimo Mattia Santoro³

Affiliations

¹ Laboratory of Angiogenesis and Cancer Metabolism, DiBio, University of Padua, Padua, Italy; Veneto Institute of Molecular Medicine (VIMM), University of Padova, Padua, Italy.
² Department of Medicine (DIMED), University of Padova, Padua, Italy; Veneto Institute of Molecular Medicine (VIMM), University of Padova, Padua, Italy.
³ Laboratory of Angiogenesis and Cancer Metabolism, DiBio, University of Padua, Padua, Italy; Veneto Institute of Molecular Medicine (VIMM), University of Padova, Padua, Italy. Electronic address: massimo.santoro@unipd.it.

Abstract

Hind limb ischemia is a useful model to assess metabolic and cellular responses. Here, we present a protocol for evaluating post-natal angiogenesis in a mouse hind limb ischemia model. We describe steps to induce a severe restriction of blood supply of the femoral artery and vein that mimics the real-life scenario observed in clinical settings. We then detail procedures for follow-up laser Doppler imaging to compare post-ischemic responses of four different mouse strains in their capacity to trigger compensatory arteriogenesis. For complete details on the use and execution of this protocol, please refer to Oberkersch et al. (2022).¹.

Keywords: Health Sciences; Metabolism; Microscopy; Model Organisms.