The polymorphism rs4705342 in the promoter of miR-143/145 is related to the risk of epithelial ovarian cancer and patient prognosis

Jian Zhao; Weiwei Zuo; Yue Zhang; Caiyun He; Wei Zhao; Tongyu Meng

doi:10.3389/fonc.2023.1122284

The polymorphism rs4705342 in the promoter of miR-143/145 is related to the risk of epithelial ovarian cancer and patient prognosis

Front Oncol. 2023 Apr 4:13:1122284. doi: 10.3389/fonc.2023.1122284. eCollection 2023.

Authors

Jian Zhao¹, Weiwei Zuo², Yue Zhang¹, Caiyun He¹, Wei Zhao³, Tongyu Meng¹

Affiliations

¹ Department of Gynecology, The People's Hospital of Shijiazhuang, Hebei Medical University, Shijiazhuang, Hebei, China.
² Department of Gynecology, The People's Hospital of Tangshan, Tangshan, Hebei, China.
³ Department of Gynecology, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei, China.

Abstract

Objective: To evaluate the effects of two genetic variants in the promoter of the miR-143/145 cluster on the risk of epithelial ovarian cancer (EOC) and the prognosis of EOC patients.

Study design: Genotypes were determined by the polymerase chain reaction and ligase detection reaction method in 563 EOC patients and 576 healthy women. The expression of miR-143 and miR-145 were detected by quantitative real-time polymerase chain reaction (qRT-PCR) in fifty-two EOC tissues.

Results: The rs4705342 CC genotype frequencies in EOC patients were higher than those in the controls (P = 0.014). Furthermore, the CC genotype of rs4705342 was associated with an advanced FIGO stage of EOC patients (P = 0.046). Patients with the rs4705342 CC genotype had shorter progression-free survival (PFS) and overall survival (OS) times than those carrying the TT genotype in multivariable analysis adjusting for clinical variables (HR = 1.30, 95% CI = 1.04-1.62, P = 0.020; HR = 1.33, 95% CI = 1.05-1.70, P = 0.020). In addition, the miR-145 levels were lower in EOC tissues with the rs4705342 CC genotype than in those with the TT genotype (P = 0.005).

Conclusion: The CC genotype of rs4705342 was related to an increased risk of EOC and poor prognosis of EOC patients, and rs4705342 may serve as a molecular marker for predicting the development of EOC and the clinical outcome of EOC patients.

Keywords: ovarian cancer; prognosis; risk; rs353292; rs4705342.

Grants and funding

This work was supported by grants from the Health and Family Planning Commission of Hebei Province, China (Grant numbers: 20221712, 20210163), and the Natural Scientific Foundation of Hebei Province (Grant number: H2022106047).