The eEF1 family of mammalian translation elongation factors

B S Negrutskii; V F Shalak; O V Novosylna; L V Porubleva; D M Lozhko; A V El'skaya

doi:10.1016/j.bbadva.2022.100067

The eEF1 family of mammalian translation elongation factors

BBA Adv. 2022 Nov 30:3:100067. doi: 10.1016/j.bbadva.2022.100067. eCollection 2023.

Authors

B S Negrutskii^{1

2

3}, V F Shalak¹, O V Novosylna¹, L V Porubleva¹, D M Lozhko¹, A V El'skaya¹

Affiliations

¹ Institute of Molecular Biology and Genetics, Acad. Zabolotnogo Str. 150, 03143 Kyiv, Ukraine.
² Aarhus Institute of Advanced Sciences, Høegh-Guldbergs Gade 6B, DK-8000 Aarhus C, Denmark.
³ Department of Molecular Biology and Genetics, Aarhus University, Universitetsbyen 81, DK-8000 Aarhus C, Denmark.

Abstract

The eEF1 family of mammalian translation elongation factors is comprised of the two variants of eEF1A (eEF1A1 and eEF1A2), and the eEF1B complex. The latter consists of eEF1Bα, eEF1Bβ, and eEF1Bγ subunits. The two eEF1A variants have similar translation activity but may differ with respect to their secondary, "moonlighting" functions. This variability is underlined by the difference in the spatial organization of eEF1A1 and eEF1A2, and also possibly by the differences in their post-translational modifications. Here, we review the data on the spatial organization and post-translation modifications of eEF1A1 and eEF1A2, and provide examples of their involvement in various processes in addition to translation. We also describe the structural models of eEF1B subunits, their organization in the subcomplexes, and the trimeric model of the entire eEF1B complex. We discuss the functional consequences of such an assembly into a complex as well as the involvement of individual subunits in non-translational processes.

Keywords: Multiprotein complex; Protein spatial organization; Translation elongation; eEF1A1; eEF1A2; eEF1B.