Cholinergic projections from the parabigeminal nucleus (Ch8) to the superior colliculus in the mouse: a combined analysis of horseradish peroxidase transport and choline acetyltransferase immunohistochemistry

Brain Res. 1986 Apr 2;370(1):144-8. doi: 10.1016/0006-8993(86)91114-5.


Choline acetyltransferase (ChAT) immunocytochemistry, acetylcholinesterase histochemistry and muscarinic receptor autoradiography demonstrated a cholinergic innervation within the superior colliculus. A method for the concurrent visualization of ChAT and transported horseradish peroxidase showed that a major extrinsic source for this cholinergic input is in the parabigeminal nucleus. We have designated these cholinergic neurons as the Ch8 cell group.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Acetylcholinesterase / metabolism
  • Animals
  • Autoradiography
  • Choline O-Acetyltransferase / metabolism
  • Cholinergic Fibers
  • Male
  • Mesencephalon / anatomy & histology*
  • Mesencephalon / metabolism
  • Mice
  • Neural Pathways / anatomy & histology
  • Neural Pathways / metabolism
  • Receptors, Muscarinic / metabolism
  • Superior Colliculi / anatomy & histology*
  • Superior Colliculi / metabolism


  • Receptors, Muscarinic
  • Choline O-Acetyltransferase
  • Acetylcholinesterase