Insight into the composite assembly process, nanofibril structure and stability of undenatured type II collagen in the presence of different types of nanocelluloses

Abstract

Four types of nanocelluloses (CNs), including cellulose nanocrystals (CNC), cellulose nanofibrils (CNF), cationic etherified nanocellulose (CCNF) and TEMPO-oxidized nanocellulose (TOCNF), were incorporated into the assembly process of undenatured type II collagen (UC-II). In the presence of CNs, the kinetics of UC-II composite assembly slightly fluctuated and the magnitude of UC-II assembly increased (from 59.93 to 66.83-85.06 %). CNC and CNF disrupted the triple helix structure of UC-II while CCNF and TOCNF had weak impact on it. Hydrogen bonding and hydrophobic interactions were dominant driving forces of UC-II/CNs, and electrostatic interactions were also involved in the fabrication of UC-II/CCNF and UC-II/TOCNF. UC-II/CNs exhibited distinct nanostructures due to the differences in shape, level, and surface group of CNs. CCNF and TOCNF contributed to the enhanced physical stability due to the increased surface charge. In addition, the thermal stability and rheological properties of UC-II/CNs were also improved. The composite assembly process, nanofibril structure and stability of UC-II in the presence of different types and levels of CNs, which was useful to develop the novel composite nanofibrils for the application in functional foods.

Keywords: Composite assembly; Nanocellulose; Undenatured type II collagen.