High-level and reusable preparation of sulforaphane by yeast cells expressing myrosinase

Lili Wang; Hamed I Hamouda; Yueyang Dong; Hong Jiang; Yongyi Quan; Yimiao Chen; Yan Liu; Jiaqi Wang; Mohamed A Balah; Xiangzhao Mao

doi:10.1016/j.fochx.2023.100668

High-level and reusable preparation of sulforaphane by yeast cells expressing myrosinase

Food Chem X. 2023 Apr 6:18:100668. doi: 10.1016/j.fochx.2023.100668. eCollection 2023 Jun 30.

Authors

Lili Wang^{1

2}, Hamed I Hamouda^{1

2

3}, Yueyang Dong^{1

2}, Hong Jiang^{1

2}, Yongyi Quan^{1

2}, Yimiao Chen^{1

2}, Yan Liu¹, Jiaqi Wang^{1

2}, Mohamed A Balah⁴, Xiangzhao Mao^{1

5

2}

Affiliations

¹ Qingdao Key Laboratory of Food Biotechnology, College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China.
² Key Laboratory of Biological Processing of Aquatic Products, China National Light Industry, Qingdao 266003, China.
³ Processes Design and Development Department, Egyptian Petroleum Research Institute, Nasr City 11727, Cairo, Egypt.
⁴ Plant Protection Department, Desert Research Center, Cairo 11753, Egypt.
⁵ Laboratory for Marine Drugs and Bioproducts of Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China.

Abstract

Myrosinase is a key tool for the fast and efficient preparation of sulforaphane which is one of the prominent natural ingredients found in brassicaceous vegetables. Here, the glucoraphanin-hydrolyzing activity of a Yarrowia lipolytica 20-8 harboring myrosinase reached 73.28 U/g dry cell weight, indicating that it had a potential application in sulforaphane preparation from glucoraphanin. An efficient and reusable process for sulforaphane preparation via myrosinase produced by Y. lipolytica 20-8 was constructed. In detail, as high as 10.32 mg sulforaphane could be produced from 1 g broccoli seed under the reaction of 40 U yeast whole-cell catalyst within 15 min with the conversion efficiency of 99.86%. Moreover, when the yeast whole-cell catalyst was reused 7 and 10 times, as high as 92.53% and 87.56% of sulforaphene yield of the initial level could be retained, respectively. Therefore, this yeast whole-cell is a potent biocatalyst for the efficient and reusable preparation of sulforaphane.

Keywords: Glucoraphanin; Myrosinase expression; Sulforaphane preparation; Yarrowia lipolytica.