Knockdown of LPCAT1 Repressed Hepatocellular Carcinoma Growth and Invasion by Targeting S100A11

Ann Clin Lab Sci. 2023 Mar;53(2):212-221.

Abstract

Objective: To explore the function of LPCAT1 in hepatocellular carcinoma progression.

Methods: Bioinformatics analysis was utilized to the data from TCGA to explore the level of LPCAT1 between normal and tumor tissues, as well as the relationship between LPCAT1 level and tumor grade and prognosis of HCC. Subsequently, we used siRNA to silence LPCAT1 in HCC cells to detect cell proliferation, migration, and invasion ability.

Results: The expression of LPCAT1 was significantly increased in HCC tissues. High LPCAT1 expression was correlated with high histologic grade and poor prognosis of HCC. In addition, silencing of LPCAT1 inhibited the proliferation, migration and invasion of liver cancer cells. Moreover, LPCAT1 knockdown suppressed S100A11 and Snail both at mRNA and protein level.

Conclusion: LPCAT1 promoted the growth, invasion and migration of HCC cells by regulating S100A11 and Snail. Therefore, LPCAT1 may serve as a potential molecular target for the diagnosis and treatment of HCC.

Keywords: Hepatocellular carcinoma; LPCAT1; growth and invasion; prognosis.

MeSH terms

  • 1-Acylglycerophosphocholine O-Acyltransferase / genetics
  • 1-Acylglycerophosphocholine O-Acyltransferase / metabolism
  • Acyltransferases / genetics
  • Carcinoma, Hepatocellular* / genetics
  • Cell Line, Tumor
  • Cell Movement / genetics
  • Cell Proliferation / genetics
  • Gene Expression Regulation, Neoplastic
  • Humans
  • Liver Neoplasms* / pathology
  • Neoplasm Invasiveness / genetics
  • Prognosis
  • S100 Proteins / genetics
  • S100 Proteins / metabolism

Substances

  • Acyltransferases
  • S100A11 protein, human
  • S100 Proteins
  • Lpcat1 protein, human
  • 1-Acylglycerophosphocholine O-Acyltransferase