Isolation of the B Cell Immune Synapse for Proteomic Analysis

Diogo M Cunha; Sara Hernández-Pérez; Pieta K Mattila

doi:10.1007/978-1-0716-3135-5_25

Isolation of the B Cell Immune Synapse for Proteomic Analysis

Methods Mol Biol. 2023:2654:393-408. doi: 10.1007/978-1-0716-3135-5_25.

Authors

Diogo M Cunha^{1

2}, Sara Hernández-Pérez^{1

2}, Pieta K Mattila^{3

4}

Affiliations

¹ Institute of Biomedicine, MediCity Research Laboratories, and InFLAMES Research Flagship, University of Turku, Turku, Finland.
² Turku Bioscience, University of Turku and Åbo Akademi University, Turku, Finland.
³ Institute of Biomedicine, MediCity Research Laboratories, and InFLAMES Research Flagship, University of Turku, Turku, Finland. pieta.mattila@utu.fi.
⁴ Turku Bioscience, University of Turku and Åbo Akademi University, Turku, Finland. pieta.mattila@utu.fi.

PMID: 37106196
DOI: 10.1007/978-1-0716-3135-5_25

Abstract

Recent technical developments have fueled increasing utilization of proteomics to gain new insights into various aspects of cellular behavior. In this chapter, we describe a method to specifically isolate immune synapses from mouse primary B cells. The method utilizes antibody-coated magnetic beads to induce the formation of the immune synapses and describes a protocol for the extraction of the cell-bead adhesions for mass spectrometry analysis. Finally, this method enables unveiling the large-scale protein content of the immune synapse.

Keywords: B cells; Cell adhesion; Immunological synapse immune synapse; Mass spectrometry; Proteomics.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
B-Lymphocytes
Mass Spectrometry
Mice
Proteins / metabolism
Proteomics* / methods
Synapses* / metabolism

Substances

Proteins