A simple procedure for the purification of neutral salt soluble type I collagen from skin

Ital J Biochem. 1986 Jan-Feb;35(1):42-8.

Abstract

Intact monomeric type I collagen was purified from fetal bovine skin by a simple and time saving procedure. Saline precipitates of mixed skin collagens, in 4 M NaCl, were extracted with a limited volume of dilute acetic acid, taken in the proportion of 1 ml per g of original wet skin; NaCl in the precipitate was not removed by dialysis. The salt concentration in the extraction medium in the above conditions, selectively solubilized type I collagen.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetates / pharmacology
  • Acetic Acid
  • Amino Acids / analysis
  • Animals
  • Cattle
  • Collagen / isolation & purification*
  • Densitometry
  • Electrophoresis, Polyacrylamide Gel
  • Fetus
  • Skin / analysis*
  • Sodium Chloride / pharmacology*
  • Solubility

Substances

  • Acetates
  • Amino Acids
  • Sodium Chloride
  • Collagen
  • Acetic Acid