Identification of an adipose tissue-resident pro-preadipocyte population

Min Chen; Soochi Kim; Liang Li; Sourav Chattopadhyay; Thomas A Rando; Brian J Feldman

doi:10.1016/j.celrep.2023.112440

Identification of an adipose tissue-resident pro-preadipocyte population

Cell Rep. 2023 May 30;42(5):112440. doi: 10.1016/j.celrep.2023.112440. Epub 2023 Apr 27.

Authors

Min Chen¹, Soochi Kim², Liang Li¹, Sourav Chattopadhyay¹, Thomas A Rando³, Brian J Feldman⁴

Affiliations

¹ Department of Pediatrics, University of California, San Francisco School of Medicine, San Francisco, CA 94158, USA.
² Department of Neurology and Neurological Sciences, Stanford University School of Medicine, Stanford, CA 94305, USA.
³ Department of Neurology and Neurological Sciences, Stanford University School of Medicine, Stanford, CA 94305, USA; Broad Stem Cell Research Center, University of California Los Angeles, Los Angeles, CA 90095, USA.
⁴ Department of Pediatrics, University of California, San Francisco School of Medicine, San Francisco, CA 94158, USA; Nutrition and Obesity Research Center, University of California, San Francisco, San Francisco, CA 94158, USA. Electronic address: brian.feldman@ucsf.edu.

Abstract

Elucidating the transitional stages that define the pathway stem cells progress through during differentiation advances our understanding of biology and fosters the identification of therapeutic opportunities. However, distinguishing progenitor cells from other cell types and placing them in an epistatic pathway is challenging. This is exemplified in the adipocyte lineage, where the stromal vascular fraction (SVF) from adipose tissue is enriched for progenitor cells but also contains heterogeneous populations of cells. Single-cell RNA sequencing (scRNA-seq) has begun to facilitate the deconvolution of cell types in the SVF, and a hierarchical structure is emerging. Here, we use scRNA-seq to discover a population of CD31^- CD45^- cells in the SVF that are distinguished by a specific expression profile. Further, we place this population on an epistatic pathway upstream of the previously defined preadipocyte population. Finally, we discover functional properties of this population with broad implications, including revealing physiological mechanisms that regulate adipogenesis.

Keywords: CD44; CP: Metabolism; CP: Stem cell research; Nocturnin; adipocyte progenitor cell; adipognesis; adipose.

Publication types

Research Support, N.I.H., Extramural

MeSH terms

Adipocytes
Adipose Tissue* / metabolism
Cell Differentiation
Stem Cells
Stromal Cells* / metabolism

Abstract

Publication types

MeSH terms

Grants and funding