Teaching through the use of animals is an integral part of veterinary education. In addition to interactions with privately owned animals, veterinary students often learn using cadavers and institutionally owned animals. Veterinary students also frequently participate in research involving animals. Animal-based research is essential for the development of therapies and techniques that improve the lives of both animals and people. To investigate the perceptions of veterinary students at the North Carolina State University, College of Veterinary Medicine (NCSU-CVM) toward the use of animals in teaching and research, an anonymous survey was provided to current and recently graduated veterinary students. The aims of the study were to 1) gain a general understanding of veterinary student perceptions surrounding the use of animals in research and teaching, 2) determine if providing simple facts about the contributions of animals to medical advancements would increase the acceptance of animal use for teaching and research, and 3) determine if general perceptions regarding the use of animals in teaching and research change over the course of completing the veterinary curriculum. Descriptive statistics and frequency distributions were calculated for applicable response types. χ² tests were used to identify factors that influenced perceptions of the use of animals in teaching and research. A change-indicator variable was created, and binary logistic regression was used to compare responses before and after completion of the educational component of the survey. Of 141 total survey respondents, 78% were accepting of the use of animals in teaching and research, with no significant difference in the overall acceptance after reading 6 facts about animal research. In addition, 24% of respondents stated that their perceptions had changed during the course of their veterinary education. Overall, veterinary students surveyed had a high acceptance of the use of animals in teaching and research.