Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1986 Jun 12-18;321(6071):674-9.
doi: 10.1038/321674a0.

Fluorescence Detection in Automated DNA Sequence Analysis

Fluorescence Detection in Automated DNA Sequence Analysis

L M Smith et al. Nature. .


We have developed a method for the partial automation of DNA sequence analysis. Fluorescence detection of the DNA fragments is accomplished by means of a fluorophore covalently attached to the oligonucleotide primer used in enzymatic DNA sequence analysis. A different coloured fluorophore is used for each of the reactions specific for the bases A, C, G and T. The reaction mixtures are combined and co-electrophoresed down a single polyacrylamide gel tube, the separated fluorescent bands of DNA are detected near the bottom of the tube, and the sequence information is acquired directly by computer.

Similar articles

See all similar articles

Cited by 236 articles

See all "Cited by" articles

Publication types

LinkOut - more resources