Fluorescence detection in automated DNA sequence analysis

Nature. 1986 Jun 12-18;321(6071):674-9. doi: 10.1038/321674a0.

Abstract

We have developed a method for the partial automation of DNA sequence analysis. Fluorescence detection of the DNA fragments is accomplished by means of a fluorophore covalently attached to the oligonucleotide primer used in enzymatic DNA sequence analysis. A different coloured fluorophore is used for each of the reactions specific for the bases A, C, G and T. The reaction mixtures are combined and co-electrophoresed down a single polyacrylamide gel tube, the separated fluorescent bands of DNA are detected near the bottom of the tube, and the sequence information is acquired directly by computer.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Automation
  • Base Sequence*
  • Computers
  • Electrophoresis, Polyacrylamide Gel / methods
  • Fluorescent Dyes*
  • Molecular Biology / instrumentation*
  • Nucleotides / analysis*
  • Spectrometry, Fluorescence / methods

Substances

  • Fluorescent Dyes
  • Nucleotides