Investigation of the Effects of Some Cardiovascular Drugs on Angiogenesis by Transgenic Zebrafish

Mediators Inflamm. 2023 Apr 24:2023:1958046. doi: 10.1155/2023/1958046. eCollection 2023.

Abstract

Introduction: Angiogenesis contributes to the pathophysiology of cardiovascular disease (CVD). Some cardiovascular drugs used in the treatment of CVD have an effect on the process of angiogenesis.

Methods: Transgenic Tg (flk1: EGFP) zebrafish embryos were used to identify the effects of some cardiovascular drugs on angiogenesis during vertebral development in vivo. Zebrafish embryos at a one-cell stage or two-cell stage were cultured with embryo medium containing cardiovascular drugs at a final solvent concentration of 0.5% (V/V) dimethyl sulfoxide (DMSO) for 24 hours in 24-well plates.

Results: We found that 6 drugs including isosorbide mononitrate, amlodipine, bisoprolol fumarate, carvedilol, irbesartan, and rosuvastatin calcium may affect angiogenesis by vascular endothelial growth factor (VEGF) signaling pathway.

Conclusion: These new findings of some cardiovascular drugs should improve the treatment of cardiovascular diseases.

MeSH terms

  • Angiogenesis Inducing Agents / pharmacology
  • Animals
  • Animals, Genetically Modified
  • Cardiovascular Agents* / pharmacology
  • Cardiovascular Diseases / drug therapy
  • Cardiovascular Diseases / pathology
  • Neovascularization, Physiologic* / drug effects
  • Vascular Endothelial Growth Factor A / metabolism
  • Zebrafish

Substances

  • Cardiovascular Agents
  • Vascular Endothelial Growth Factor A
  • Angiogenesis Inducing Agents