LBP1C-2 from Lycium barbarum maintains skeletal muscle satellite cell pool by interaction with FGFR1

Jiao Meng; Zhenyu Lv; Xia Chen; Chuanxin Sun; Can Jin; Kan Ding; Chang Chen

doi:10.1016/j.isci.2023.106573

LBP1C-2 from Lycium barbarum maintains skeletal muscle satellite cell pool by interaction with FGFR1

iScience. 2023 Apr 6;26(5):106573. doi: 10.1016/j.isci.2023.106573. eCollection 2023 May 19.

Authors

Jiao Meng¹, Zhenyu Lv^{1

2}, Xia Chen³, Chuanxin Sun¹, Can Jin³, Kan Ding^{3

2

4}, Chang Chen^{1

2

5}

Affiliations

¹ National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China.
² University of Chinese Academy of Sciences, Beijing 100049, China.
³ CAS Key Laboratory of Receptor Research, State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.
⁴ Zhongshan Institute for Drug Discovery, Shanghai Institute of Materia Medica, Chinese Academy of Science, SSIP Healthcare and Medicine Demonstration Zone, Zhongshan, Guangdong 528400, China.
⁵ Beijing Institute for Brain Disorders, Capital Medical University, Beijing 100069, China.

Abstract

Muscle stem cells, called satellite cells (SCs), are employed to repair and rebuild muscle. SC-based therapeutic strategies are urgently needed. In this study, we showed that Lycium barbarum extract (LBE) improved the number of SCs and enhanced muscle regeneration by promoting SC activation and self-renewal in both adult and aging mice. L. barbarum polysaccharide (LBP), the main component of LBE, also played a similar role. More importantly, LBP1C-2, a homogeneous polysaccharide isolated from LBP, was uncovered to be an active component in regulating SC function. Mechanism study revealed that LBP1C-2 might bind to FGFR1 to activate SCs and promote SC self-renewal through Spry1 upregulation. This might be the first study to show that LBE participated in the regulation of SCs, and the active components and targets of LBE were identified. This study lays a theoretical foundation for the medicinal or auxiliary medicinal use of L. barbarum in skeletal muscle.

Keywords: Biological sciences; Cell biology; Molecular biology; Stem cells research.