High-Efficiency Single-Cell Electrical Impedance Spectroscopy

Methods Mol Biol. 2023;2644:81-97. doi: 10.1007/978-1-0716-3052-5_6.


Single-cell impedance measurement is label free and noninvasive in characterizing the electrical properties of single cells. At present, though widely used for impedance measurement, electrical impedance flow cytometry (IFC) and electrical impedance spectroscopy (EIS) are used alone for most microfluidic chips. Here, we describe high-efficiency single-cell electrical impedance spectroscopy, which combines in one chip the IFC and EIS techniques for high-efficiency single-cell electrical property measurement. We envision that the strategy of combining IFC and EIS provides a new thought in the efforts to enhance the efficiency of electrical property measurement for single cells.

Keywords: Electric impedance spectroscopy; Electrical property; Impedance flow cytometry; Microfluidics; Single cell analysis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Dielectric Spectroscopy*
  • Electric Impedance
  • Flow Cytometry / methods
  • Microfluidics / methods
  • Single-Cell Analysis* / methods