Bombyx mori nucleopolyhedrovirus induces BmFABP1 downregulation to promote viral proliferation

Insect Sci. 2023 Dec;30(6):1595-1606. doi: 10.1111/1744-7917.13200. Epub 2023 May 5.


Fatty acid binding proteins (FABPs) play an important role as endogenous cytoprotectants. However, studies on FABPs in invertebrates are scarce. Previously, we discovered Bombyx mori fatty acid binding protein 1 (BmFABP1) through co-immunoprecipitation. Here, we cloned and identified BmFABP1 from BmN cells. The results of immunofluorescence indicated that BmFABP1 was localized in the cytoplasm. The tissue expression profile of silkworms showed that BmFABP1 was expressed in all tissues except hemocytes. The expression level of BmFABP1 gradually decreases in BmN cells and B. mori larvae after infection with B. mori nucleopolyhedrovirus (BmNPV). Upregulation of BmFABP1 expression through overexpression or WY14643 treatment significantly inhibited the replication of BmNPV, while downregulation of BmFABP1 expression by RNA interference promoted the replication of BmNPV. The same results were obtained in experiments on silkworm larvae. These results suggest that BmNPV induces BmFABP1 downregulation to promote its proliferation and that BmFABP1 has a potential anti-BmNPV role. This is the first report on the antiviral effect of BmFABP1 in silkworms and provides new insights into the study of the FABP protein family. Also, it is important to study BmNPV resistance in silkworms to breed transgenic silkworms with BmNPV resistance.

Keywords: BmFABP1; BmNPV; Bombyx mori; antiviral.

MeSH terms

  • Animals
  • Bombyx* / metabolism
  • Cell Proliferation
  • Down-Regulation
  • Insect Proteins / genetics
  • Insect Proteins / metabolism
  • Larva / metabolism
  • Nucleopolyhedroviruses* / physiology


  • Insect Proteins

Supplementary concepts

  • Bombyx mori nucleopolyhedrovirus