Roles of adenine methylation in the physiology of Lacticaseibacillus paracasei

Jie Zhao; Meng Zhang; Wenyan Hui; Yue Zhang; Jing Wang; Shaojing Wang; Lai-Yu Kwok; Jian Kong; Heping Zhang; Wenyi Zhang

doi:10.1038/s41467-023-38291-1

Roles of adenine methylation in the physiology of Lacticaseibacillus paracasei

Nat Commun. 2023 May 6;14(1):2635. doi: 10.1038/s41467-023-38291-1.

Authors

Jie Zhao^#^{1

2

3}, Meng Zhang^#^{1

2

3}, Wenyan Hui^{1

2

3}, Yue Zhang^{1

2

3}, Jing Wang^{1

2

3}, Shaojing Wang⁴, Lai-Yu Kwok^{1

2

3}, Jian Kong⁵, Heping Zhang^{1

2

3}, Wenyi Zhang^{6

7

8}

Affiliations

¹ Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University, Hohhot, 010018, China.
² Key Laboratory of Dairy Products Processing, Ministry of Agriculture and Rural Affairs, Inner Mongolia Agricultural University, Hohhot, 010018, China.
³ Inner Mongolia Key Laboratory of Dairy Biotechnology and Engineering, Inner Mongolia Agricultural University, Hohhot, 010018, China.
⁴ Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Sciences, Nankai University, Tianjin, 300071, China.
⁵ State Key Laboratory of Microbial Technology, Shandong University, Qingdao, 266237, China.
⁶ Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University, Hohhot, 010018, China. zhangwenyizi@163.com.
⁷ Key Laboratory of Dairy Products Processing, Ministry of Agriculture and Rural Affairs, Inner Mongolia Agricultural University, Hohhot, 010018, China. zhangwenyizi@163.com.
⁸ Inner Mongolia Key Laboratory of Dairy Biotechnology and Engineering, Inner Mongolia Agricultural University, Hohhot, 010018, China. zhangwenyizi@163.com.

^# Contributed equally.

Abstract

Lacticaseibacillus paracasei is an economically important bacterial species, used in the food industry and as a probiotic. Here, we investigate the roles of N6-methyladenine (6mA) modification in L. paracasei using multi-omics and high-throughput chromosome conformation capture (Hi-C) analyses. The distribution of 6mA-modified sites varies across the genomes of 28 strains, and appears to be enriched near genes involved in carbohydrate metabolism. A pglX mutant, defective in 6mA modification, shows transcriptomic alterations but only modest changes in growth and genomic spatial organization.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adenine / metabolism
DNA Methylation
Genomics
Lacticaseibacillus paracasei*
Lacticaseibacillus*

Substances

Adenine