Repetition plasticity is a ubiquitous property of sensory systems in which repetitive sensation causes either a decrease ("repetition suppression", i.e. "adaptation") or increase ("repetition enhancement", i.e. "facilitation") in the amplitude of neural responses. Timescales of repetition plasticity for sensory neurons typically span milliseconds to tens of seconds, with longer durations for cortical vs subcortical regions. Here, we used 2-photon (2P) imaging to study repetition plasticity in mouse primary auditory cortex (A1) layer 2/3 (L2/3) during the presentation of spectrotemporally randomized pure-tone frequencies. Our study revealed subpopulations of neurons with repetition plasticity for equiprobable frequencies spaced minutes apart over a 20-minute period. We found both repetition suppression and enhancement in individual neurons and on average across populations. Each neuron tended to show repetition plasticity for 1-2 pure-tone frequencies near the neuron's best frequency. Moreover, we found correlated changes in neural response amplitude and latency across stimulus repetitions. Together, our results highlight cortical specialization for pattern recognition over long timescales in complex acoustic sequences.