Immobilization of lipase on hydrophobic MOF synthesized simultaneously with oleic acid and application in hydrolysis of natural oils for improving unsaturated fatty acid production

Geling Kuang; Zichen Wang; Xiuyan Luo; Zixin Geng; Jiandong Cui; Muhammad Bilal; Ziyuan Wang; Shiru Jia

doi:10.1016/j.ijbiomac.2023.124807

Immobilization of lipase on hydrophobic MOF synthesized simultaneously with oleic acid and application in hydrolysis of natural oils for improving unsaturated fatty acid production

Int J Biol Macromol. 2023 Jul 1;242(Pt 2):124807. doi: 10.1016/j.ijbiomac.2023.124807. Epub 2023 May 11.

Authors

Geling Kuang¹, Zichen Wang¹, Xiuyan Luo¹, Zixin Geng¹, Jiandong Cui², Muhammad Bilal³, Ziyuan Wang⁴, Shiru Jia¹

Affiliations

¹ State Key Laboratory of Food Nutrition and Safety, Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin University of Science and Technology, Tianjin Economic and Technological Development Area (TEDA), No 29, 13(th), Avenue, Tianjin 300457, PR China.
² State Key Laboratory of Food Nutrition and Safety, Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin University of Science and Technology, Tianjin Economic and Technological Development Area (TEDA), No 29, 13(th), Avenue, Tianjin 300457, PR China. Electronic address: jdcui@tust.edu.cn.
³ Institute of Chemical Technology and Engineering, Faculty of Chemical Technology, Poznan University of Technology, Berdychowo 4, 60695 Poznan, Poland.
⁴ State Key Laboratory of Food Nutrition and Safety, Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin University of Science and Technology, Tianjin Economic and Technological Development Area (TEDA), No 29, 13(th), Avenue, Tianjin 300457, PR China. Electronic address: Ziyuan@edu.edu.cn.

PMID: 37178887
DOI: 10.1016/j.ijbiomac.2023.124807

Abstract

The hydrolysis of natural oils (vegetable oils and fats) by lipase has significant applications in food and medicine. However, free lipases are usually sensitive to temperature, pH and chemical reagents in aqueous solutions, which hinders their widespread industrial application. Excitingly, immobilized lipases have been widely reported to overcome these problems. Herein, inspired by lipase interface activation, a hydrophobic Zr-MOF (UiO-66-NH₂-OA) with oleic acid was synthesized for the first time in an emulsion consisting of oleic acid and water, and the Aspergillus oryzae lipase (AOL) was immobilized onto the UiO-66-NH₂-OA through hydrophobic interaction and electrostatic interaction to obtain immobilized lipase (AOL/UiO-66-NH₂-OA). ¹H NMR and FT-IR data indicated that oleic acid was conjugated with the 2-amino-1,4-benzene dicarboxylate (BDC-NH₂) by amidation reaction. As a result, the V_max and K_cat values of AOL/UiO-66-NH₂-OA were 179.61 μM﹒min^-1 and 8.27 s^-1, which were 8.56 and 12.92 times higher than those of the free enzyme, respectively, due to the interfacial activation. After treated at 70 °C for 120 min, the immobilized lipase maintained 52 % of its original activity, but free AOL only retained 15 %. Significantly, the yield of fatty acids by the immobilized lipase reached 98.3 % and still exceeded 82 % after seven times of recycling.

Keywords: Hydrolysis of soybean oil; Hydrophobic Zr-MOF; Interface activation; Lipase; Oleic acid.

MeSH terms

Enzymes, Immobilized / chemistry
Fatty Acids, Unsaturated
Hydrolysis
Hydrophobic and Hydrophilic Interactions
Lipase* / chemistry
Oleic Acid*
Plant Oils / chemistry
Spectroscopy, Fourier Transform Infrared
Water

Substances

Lipase
UiO-66
Oleic Acid
Enzymes, Immobilized
Plant Oils
Fatty Acids, Unsaturated
Water