Cell-free synthesis of delta-(L-alpha-aminoadipyl)-L-cysteine, the first intermediate of penicillin and cephalosporin biosynthesis

Biochem Biophys Res Commun. 1986 May 29;137(1):528-35. doi: 10.1016/0006-291x(86)91242-8.

Abstract

delta-(L-alpha-aminoadipyl)-L-cysteine synthetase (LL-AC synthetase) activity has been found in extracts of Cephalosporium acremonium C-10. The enzyme extract carries out a linear synthesis of LL-AC from its constituent amino acids for at least 6 hours. The reaction is dependent on active enzyme, time, L-alpha-aminoadipate, L-cysteine, ATP and Mn2+ or Mg2+. The activity is stabilized by glycerol.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Acremonium / metabolism*
  • Adenosine Triphosphate / metabolism
  • Cell-Free System
  • Cephalosporins / biosynthesis*
  • Chromatography, High Pressure Liquid
  • Dipeptides / biosynthesis*
  • Kinetics
  • Magnesium / metabolism
  • Manganese / metabolism
  • Penicillins / biosynthesis*
  • Temperature

Substances

  • 5-(2-aminoadipyl)cysteine
  • Cephalosporins
  • Dipeptides
  • Penicillins
  • Manganese
  • Adenosine Triphosphate
  • Magnesium