Whole genome sequencing of a novel chitinolytic Streptomyces sp. RB7AG reveals it's chitosan production potential: optimization of the process through Taguchi experimental design

Subhransu Sekhar Behera; Suchismita Nivedita; Smrutiranjan Das; Himadri Tanaya Behera; Abhik Mojumdar; Lopamudra Ray

doi:10.1007/s13205-023-03613-z

Whole genome sequencing of a novel chitinolytic Streptomyces sp. RB7AG reveals it's chitosan production potential: optimization of the process through Taguchi experimental design

3 Biotech. 2023 Jun;13(6):198. doi: 10.1007/s13205-023-03613-z. Epub 2023 May 18.

Authors

Subhransu Sekhar Behera¹, Suchismita Nivedita¹, Smrutiranjan Das¹, Himadri Tanaya Behera¹, Abhik Mojumdar¹, Lopamudra Ray^{1

2}

Affiliations

¹ Odisha Bhubaneswar, India School of Biotechnology, Kalinga Institute of Industrial Technology (KIIT), Deemed to be University.
² Odisha Bhubaneswar, India School of Law, KIIT Deemed to be University.

PMID: 37215370
PMCID: PMC10192506 (available on 2024-06-01)
DOI: 10.1007/s13205-023-03613-z

Abstract

This study reports about the De novo whole genome sequencing and analysis of a bacterial isolate Streptomyces sp. Strain. RB7AG, isolated from the sediments of Chilika Lake, Odisha, India. The genome report in this paper highlights a size of 7,708,681 bp and a GC content of 72.3%. It also consists of 7274 coding sequence, 66 tRNA and 4 rRNA. Furthermore, carbohydrate active enzyme analysis revealed that the strain RB7AG has 127 glycoside hydrolase family genes, which is well known for hydrolysis of glycosidic bond in complex sugars. Thus, exploiting these microorganisms for the production of chitosan can be an appropriate waste disposal method of choice. Chitosan being an important biomolecule that has various industrial applications. Hence, the study also sought to improve the culture conditions for the Streptomyces sp. strain RB7AG for generation, recovery, and characterization of chitosan. Utilizing the isolate, various low-cost nitrogen sources, including peptone, yeast extract, ammonium chloride, urea along with pH, media, metal ions and surfactant were assessed for chitosan synthesis. In this context, traditional methods such as One Factor One Time are more time consuming and expensive too. The current work aims to establish a methodology to optimize the degradation of chitin by the chitinolytic Streptomyces sp. strain RB7AG, isolated from lake sediment for the production of chitosan. More than one factor was considered at the time of experiments, and the knowledge was integrated into Taguchi statistical design to determine the contribution of the most important factors required to achieve the desired end product i.e. chitosan. Highest chitosan production (2.188 µg/ml) was observed in MSM media, 1.0% NaCl (w/v), 0.5% Yeast extract, 1% Ca²⁺ and 0.1% Tween 80 at pH 9. The whole genome analysis of RB7AG would help in determining the mechanism involved in the breakdown activity.

Supplementary information: The online version contains supplementary material available at 10.1007/s13205-023-03613-z.

Keywords: Chitin; Chitin deacetyalse; Chitosan; Whole genome sequence.

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