Evaluation of DNA damage in obese children using the chromatin dispersion test

Biotech Histochem. 2023 Nov;98(6):396-400. doi: 10.1080/10520295.2023.2210848. Epub 2023 May 23.


Childhood obesity predicts adult obesity and may increase the lifetime risk of adverse health outcomes. Obesity is characterized by oxidative stress that can induce DNA damage; however, studies of childhood and adolescent obesity are scarce. We investigated DNA damage due to obesity in Mexican children using the chromatin dispersion test (CDT). We evaluated DNA damage to peripheral lymphocytes of 32 children grouped according to body mass index as normal weight (controls), overweight and obese groups using guidelines from the Centers for Disease Control (CDC). We found that the greatest DNA damage occurred in cells of obese children compared to normal weight and overweight children. Our findings support preventive action to obviate adverse health outcomes due to obesity.

Keywords: Childhood; DNA damage; chromatin dispersion test; human; obesity; weight.

MeSH terms

  • Adolescent
  • Adult
  • Body Mass Index
  • Child
  • Chromatin / genetics
  • DNA Damage
  • Humans
  • Overweight
  • Pediatric Obesity* / genetics


  • Chromatin