Reduction of methionine sulfoxide to methionine by Escherichia coli

J Bacteriol. 1979 Jul;139(1):161-4. doi: 10.1128/jb.139.1.161-164.1979.

Abstract

L-Methionine-dl-sulfoxide can support the growth of an Escherichia coli methionine auxotroph, suggesting the presence of an enzyme(s) capable of reducing the sulfoxide to methionine. This was verified by showing that a cell-free extract of E. coli catalyzes the conversion of methionine sulfoxide to methionine. This reaction required reduced nicotinamide adenine dinucleotide phosphate and a generating system for this compound. The specific activity of the enzyme increased during logarithmic growth and was maximal when the culture attained a density of about 10(9) cells per ml.

MeSH terms

  • Escherichia coli / enzymology
  • Escherichia coli / growth & development
  • Escherichia coli / metabolism*
  • Glucosephosphates / metabolism
  • Kinetics
  • Methionine / analogs & derivatives*
  • Methionine / metabolism
  • NADP / pharmacology
  • Sulfoxides / metabolism*

Substances

  • Glucosephosphates
  • Sulfoxides
  • NADP
  • Methionine