Purpose To investigate aging and fracture resistance of screw-retained implant-supported single crowns (iSCs) fabricated from lithium disilicate containing virgilite (VLD). Materials and Methods iSCs were fabricated from VLD (CEREC Tessera, Dentsply Sirona) and lithium disilicate (control; n=8, e.max CAD, Ivoclar) and bonded to a Ti-base abutment. VLD crowns were luted either with (VLDc, n=8) or without (VLDw, n=8) a coupling agent. iSCs were cleaned, sterilized, and screw-retained on implants. Fracture force was determined after thermal cycling and mechanical loading (TCML). Results All crowns survived TCML. No significant differences in mean fracture forces were identified between VLDc 1583 N, VLDw 1694 N, and control 1797 N (P=0.639 ANOVA, P=1.000 Bonferroni). Conclusion Screw-retained iSCs fabricated from VLD provide acceptable stability, which is independent on the usage of a coupling agent for bonding to Ti-base abutments and sterilization. Int J Prosthodont 2023. doi: 10.11607/ijp.8369.