Distribution, metabolism, and toxicity of antimony species in wistar rats. A bio-analytical approach

Yasumitsu Ogra; Nicole Roldán; Marcelo Verdugo; Alexis A Gonzalez; Noriyuki Suzuki; Waldo Quiroz

doi:10.1016/j.etap.2023.104160

Distribution, metabolism, and toxicity of antimony species in wistar rats. A bio-analytical approach

Environ Toxicol Pharmacol. 2023 Jun:100:104160. doi: 10.1016/j.etap.2023.104160. Epub 2023 May 24.

Authors

Yasumitsu Ogra¹, Nicole Roldán², Marcelo Verdugo³, Alexis A Gonzalez⁴, Noriyuki Suzuki⁵, Waldo Quiroz⁶

Affiliations

¹ Laboratory of Toxicology and Environmental Health, Graduate School of Pharmaceutical Sciences, Chiba University, Japan. Electronic address: ogra@chiba-u.jp.
² Laboratory of Toxicology and Environmental Health, Graduate School of Pharmaceutical Sciences, Chiba University, Japan; Laboratorio de Química Analítica y Ambiental, Instituto de Química, Pontificia Universidad Católica de Valparaíso, Chile; Laboratorio de Química Biológica, Instituto de Química, Pontificia Universidad Católica de Valparaíso, Chile.
³ Laboratorio de Química Analítica y Ambiental, Departamento de Química y Bioquímica, Facultad de Ciencias, Universidad de Valparaíso, Valparaíso, Chile.
⁴ Laboratorio de Química Biológica, Instituto de Química, Pontificia Universidad Católica de Valparaíso, Chile.
⁵ Laboratory of Toxicology and Environmental Health, Graduate School of Pharmaceutical Sciences, Chiba University, Japan.
⁶ Laboratorio de Química Analítica y Ambiental, Instituto de Química, Pontificia Universidad Católica de Valparaíso, Chile.

PMID: 37236494
DOI: 10.1016/j.etap.2023.104160

Abstract

This work studied the distribution, reactivity, and biological effects of pentavalent or trivalent antimony (Sb(V), Sb(III)) and N-methylglucamine antimonate (NMG-Sb(V)) in Wistar Rats. The expression of fibrosis genes such as α - SMA, PAI-1, and CTGF were determined in Liver, and Kidney tissues. Wistar rats were treated with different concentrations of Sb(V), Sb(III), As(V) and As(III), and MA via intra-peritoneal injections. The results indicated a noteworthy elevation in mRNA levels of plasminogen activator 1 (PAI-1) in the kidneys of rats that were injected. The main accumulation site for Sb(V) was observed to be the liver, from which it is primarily excreted in its reduced form (Sb(III)) through the urine. The generation of Sb(III) in the kidneys has been found to induce damage through the expression of α-SMA and CTGF, and also lead to a higher creatinine clearance compared to As(III).

Keywords: CTGF; Kidney; PAI-1; Sb(III) distribution; Sb(III) reduction; Urine; α- SMA.

MeSH terms

Animals
Antimony* / metabolism
Antimony* / toxicity
Meglumine Antimoniate
Plasminogen Activator Inhibitor 1*
Rats
Rats, Wistar

Substances

Antimony
Plasminogen Activator Inhibitor 1
Meglumine Antimoniate