Development of two immunochromatographic test strips based on signal amplification and selenium nanoparticles for the rapid detection of T-2 mycotoxin

Xianrui Chen; Zhipeng Gao; Tingting Long; Jianhua Xie; Xiujiang Li; Zhibing Huang

doi:10.1016/j.foodchem.2023.136419

Development of two immunochromatographic test strips based on signal amplification and selenium nanoparticles for the rapid detection of T-2 mycotoxin

Food Chem. 2023 Oct 30:424:136419. doi: 10.1016/j.foodchem.2023.136419. Epub 2023 May 22.

Authors

Xianrui Chen¹, Zhipeng Gao², Tingting Long², Jianhua Xie³, Xiujiang Li⁴, Zhibing Huang⁵

Affiliations

¹ State Key Laboratory of Food Science and Resources, Nanchang University, No. 235 Nanjing East Road, Nanchang 330047, China; Jiangzhong Pharmaceutical Co., Ltd., Nanchang 330096, China; Sino-German Joint Research Institute, Nanchang University, No. 235 Nanjing East Road, Nanchang 330047, China.
² State Key Laboratory of Food Science and Resources, Nanchang University, No. 235 Nanjing East Road, Nanchang 330047, China; Sino-German Joint Research Institute, Nanchang University, No. 235 Nanjing East Road, Nanchang 330047, China.
³ State Key Laboratory of Food Science and Resources, Nanchang University, No. 235 Nanjing East Road, Nanchang 330047, China.
⁴ The First Affiliated Hospital of Nanchang University, Nanchang University, No.17 Yongwai Main Street, Nanjing West Road, Nanchang 330006, China.
⁵ State Key Laboratory of Food Science and Resources, Nanchang University, No. 235 Nanjing East Road, Nanchang 330047, China; Sino-German Joint Research Institute, Nanchang University, No. 235 Nanjing East Road, Nanchang 330047, China. Electronic address: hzbchem@ncu.edu.cn.

PMID: 37244190
DOI: 10.1016/j.foodchem.2023.136419

Abstract

Conventional immunochromatographic test strips (ICSs) based on gold nanoparticle (AuNP) probes offer limited sensitivity. Here, AuNPs were separately labeled with monoclonal or secondary antibodies (MAb or SAb). In addition, spherical, homogeneously dispersed, and stable selenium nanoparticles (SeNPs) were also synthesized. By optimizing the preparation parameters, two ICSs based on the dual AuNP signal amplification (Duo-ICS) or SeNPs (Se-ICS) were developed for the rapid detection of T-2 mycotoxin. The detection sensitivities of the Duo-ICS and Se-ICS assays for T-2 were 1 ng/mL and 0.25 ng/mL, respectively, which were 3-fold and 15-fold more sensitive, respectively, than a conventional ICS. Furthermore, the ICSs were applied in the detection of T-2 in cereals, which requires higher sensitivity. Our findings indicate that both ICS systems can be used for rapid, sensitive, and specific detection of T-2 toxin in cereals and potentially other sample types.

Keywords: Cereal; Gold nanoparticle; Immunochromatographic test strip; Selenium nanoparticle; T-2 toxin.

MeSH terms

Antibodies, Monoclonal
Chromatography, Affinity / methods
Gold / chemistry
Limit of Detection
Metal Nanoparticles* / chemistry
Mycotoxins*
Selenium*

Substances

Gold
Mycotoxins
Selenium
Antibodies, Monoclonal