Lmpt regulates the function of Drosophila muscle by acting as a repressor of Wnt signaling

Gene. 2023 Aug 5:876:147514. doi: 10.1016/j.gene.2023.147514. Epub 2023 May 26.


Background: LIM domain is considered to be important in mediating protein-protein interactions, and members of the LIM protein family can co-regulate tissue-specific gene expression by interacting with different transcription factors. However, its exact function in vivo remains unclear. Our study demonstrates that the LIM protein family member Lmpt may act as a cofactor that interacts with other transcription factors to regulate cellular functions.

Methods: In this study, we generated Lmpt knockdown Drosophila (Lmpt-KD) using the UAS-Gal4 system. We assessed the lifespan and motility of Lmpt-KD Drosophila and analyzed the expression of muscle-related and metabolism-related genes using qRT-PCR. Additionally, we utilized Western blot and Top-Flash luciferase reporter assay to evaluate the level of the Wnt signaling pathway.

Results: Our study revealed that knockdown of the Lmpt gene in Drosophila resulted in a shortened lifespan and reduced motility. We also observed a significant increase in oxidative free radicals in the fly gut. Furthermore, qRT-PCR analysis indicated that knockdown of Lmpt led to decreased expression of muscle-related and metabolism-related genes in Drosophila, suggesting that Lmpt plays a crucial role in maintaining muscle and metabolic functions. Finally, we found that reduction of Lmpt significantly upregulated the expression of Wnt signaling pathway proteins.

Conclusion: Our results demonstrate that Lmpt is essential for motility and survival in Drosophila and acts as a repressor in Wnt signaling.

Keywords: Drosophila; LIM domain; Lmpt; Motility; Wnt/Wg signaling.

MeSH terms

  • Animals
  • Drosophila Proteins* / genetics
  • Drosophila* / genetics
  • Muscles / metabolism
  • Transcription Factors / genetics
  • Wnt Signaling Pathway
  • Wnt1 Protein / genetics


  • Drosophila Proteins
  • Wnt1 Protein
  • Transcription Factors