Guar gum modified tilmicosin-loaded sodium alginate/gelatin composite nanogels for effective therapy of porcine proliferative enteritis caused by Lawsonia intracellularis

Wanhe Luo; Kuiyu Meng; Yiqing Zhao; Jinhuan Liu; Dongmei Chen; Chunyan Xu; Samah Attia Algharib; Ali Sobhy Dawood; Shuyu Xie

doi:10.1016/j.ijbiomac.2023.125084

Guar gum modified tilmicosin-loaded sodium alginate/gelatin composite nanogels for effective therapy of porcine proliferative enteritis caused by Lawsonia intracellularis

Int J Biol Macromol. 2023 Jul 1;242(Pt 3):125084. doi: 10.1016/j.ijbiomac.2023.125084. Epub 2023 May 27.

Authors

Wanhe Luo¹, Kuiyu Meng², Yiqing Zhao², Jinhuan Liu³, Dongmei Chen⁴, Chunyan Xu³, Samah Attia Algharib⁵, Ali Sobhy Dawood⁶, Shuyu Xie⁷

Affiliations

¹ National Reference Laboratory of Veterinary Drug Residues (HZAU) and MARA Key Laboratory for Detection of Veterinary Drug Residues, Huazhong Agricultural University, Wuhan, Hubei 430070, China; Engineering Laboratory for Tarim Animal Diseases Diagnosis and Control, College of Animal Science and Technology, Tarim University, Alar, Xinjiang 843300, China.
² National Reference Laboratory of Veterinary Drug Residues (HZAU) and MARA Key Laboratory for Detection of Veterinary Drug Residues, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
³ Engineering Laboratory for Tarim Animal Diseases Diagnosis and Control, College of Animal Science and Technology, Tarim University, Alar, Xinjiang 843300, China.
⁴ MARA Laboratory for Risk Assessment of Quality and Safety of Livestock and Poultry Products, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
⁵ National Reference Laboratory of Veterinary Drug Residues (HZAU) and MARA Key Laboratory for Detection of Veterinary Drug Residues, Huazhong Agricultural University, Wuhan, Hubei 430070, China; Department of Clinical Pathology, Faculty of Veterinary Medicine, Benha University, Moshtohor, Toukh 13736, QG, Egypt.
⁶ National Reference Laboratory of Veterinary Drug Residues (HZAU) and MARA Key Laboratory for Detection of Veterinary Drug Residues, Huazhong Agricultural University, Wuhan, Hubei 430070, China; Medicine and Infectious Diseases Department, Faculty of Veterinary Medicine, University of Sadat City, 32897, Egypt.
⁷ National Reference Laboratory of Veterinary Drug Residues (HZAU) and MARA Key Laboratory for Detection of Veterinary Drug Residues, Huazhong Agricultural University, Wuhan, Hubei 430070, China. Electronic address: snxsy1@126.com.

PMID: 37245769
DOI: 10.1016/j.ijbiomac.2023.125084

Abstract

In order to overcome the treatment difficulty of Lawsonia intracellularis (L.intracellularis) using antibiotics, the tilmicosin (TIL)-loaded sodium alginate (SA)/gelatin composite nanogels modified with bioadhesive substances were designed. The optimized nanogels were prepared by electrostatic interaction between SA and gelatin at a mass ratio of 1:1 and CaCl₂ as an ionic crosslinker and further modified with guar gum (GG). The optimized TIL-nanogels modified with GG had a uniform spherical shape with a diameter of 18.2 ± 0.3 nm, LC of 29.4 ± 0.2 %, EE of 70.4 ± 1.6 %, PDI of 0.30 ± 0.04, and ZP of -32.2 ± 0.5 mv. The FTIR, DSC, and PXRD showed that GG was covered on the surface of TIL-nanogels in a pattern of staggered arrangements. The TIL-nanogels modified with GG had the strongest adhesive strength amongst those with I-carrageenan and locust bean gum and the plain nanogels, and thus significantly enhanced the cellular uptake and accumulation of TIL via clathrin-mediated endocytosis. It exhibited an increased therapeutic effect against L.intracellularis in vitro and in vivo. This study will provide guidance for developing nanogels for intracellular bacterial infection treatment.

Keywords: Guar gum (GG); Intracellular delivery; Lawsonia intracellularis (L.intracellularis); Nanogels; Tilmicosin (TIL).

MeSH terms

Alginates
Animals
Enteritis* / microbiology
Gastroenteritis*
Gelatin
Lawsonia Bacteria*
Nanogels
Swine

Substances

guar gum
Nanogels
Gelatin
tilmicosin
Alginates