Imatinib-Functionalized Galactose Hydrogels Loaded with Nanohydroxyapatite as a Drug Delivery System for Osteosarcoma: In Vitro Studies

Paulina Sobierajska; Benita Wiatrak; Paulina Jawien; Maciej Janeczek; Katarzyna Wiglusz; Adam Szeląg; Rafal J Wiglusz

doi:10.1021/acsomega.3c00986

Imatinib-Functionalized Galactose Hydrogels Loaded with Nanohydroxyapatite as a Drug Delivery System for Osteosarcoma: In Vitro Studies

ACS Omega. 2023 May 9;8(20):17891-17900. doi: 10.1021/acsomega.3c00986. eCollection 2023 May 23.

Authors

Paulina Sobierajska¹, Benita Wiatrak², Paulina Jawien³, Maciej Janeczek³, Katarzyna Wiglusz⁴, Adam Szeląg², Rafal J Wiglusz¹

Affiliations

¹ Institute of Low Temperature and Structure Research, Polish Academy of Sciences, Okolna 2, Wroclaw 50-422, Poland.
² Department of Pharmacology, Wroclaw Medical University, Mikulicza-Radeckiego 2, Wroclaw 50-345, Poland.
³ Department of Biostructure and Animal Physiology, Wroclaw University of Environmental and Life Sciences, Norwida 25/27, 50-375 Wroclaw, Poland.
⁴ Department of Basic Chemical Sciences, Faculty of Pharmacy, Wroclaw Medical University, Borowska 211 A, 50-566 Wroclaw, Poland.

Abstract

This study reports an impact of structure (XRPD, FT-IR) and surface morphology (SEM-EDS) of imatinib-functionalized galactose hydrogels, loaded and unloaded with nHAp, on osteosarcoma cell (Saos-2 and U-2OS) viability, levels of free oxygen radicals, and nitric oxide, levels of BCL-2, p53, and caspase 3 and 9, as well as glycoprotein-P activity. It was investigated how the rough surface of the crystalline hydroxyapatite-modified hydrogel affected amorphous imatinib (IM) release. The imatinib drug effect on cell cultures has been demonstrated in different forms of administration-directly to the culture or the hydrogels. Administration of IM and hydrogel composites could be expected to reduce the risk of multidrug resistance development by inhibiting P_gp.