Research progress in methods for detecting neutralizing antibodies against SARS-CoV-2

Chunxia Chen; Jiahui Liang; Hangzhan Hu; Xiaoquan Li; Li Wang; Zhizeng Wang

doi:10.1016/j.ab.2023.115199

Research progress in methods for detecting neutralizing antibodies against SARS-CoV-2

Anal Biochem. 2023 Jul 15:673:115199. doi: 10.1016/j.ab.2023.115199. Epub 2023 May 29.

Authors

Chunxia Chen¹, Jiahui Liang¹, Hangzhan Hu², Xiaoquan Li³, Li Wang⁴, Zhizeng Wang⁵

Affiliations

¹ Joint National Laboratory for Antibody Drug Engineering, Clinical Laboratory of the First Affiliated Hospital, Henan University, Kaifeng, 475004, China.
² Joint National Laboratory for Antibody Drug Engineering, Clinical Laboratory of the First Affiliated Hospital, Henan University, Kaifeng, 475004, China; Heze Municipal Hospital, Heze, 274000, China.
³ Heze Municipal Hospital, Heze, 274000, China.
⁴ Department of Clinical Laboratory, The First Affiliated Hospital of Henan University, Kaifeng, 475004, China. Electronic address: wangli851217@163.com.
⁵ Joint National Laboratory for Antibody Drug Engineering, Clinical Laboratory of the First Affiliated Hospital, Henan University, Kaifeng, 475004, China; Center of Smart Laboratory and Molecular Medicine, School of Medicine, Chongqing University, Chongqing, 400044, China. Electronic address: wzhzeng@126.com.

Abstract

The emergence of SARS-CoV-2 has seriously affected the lives of people worldwide. Clarifying the attenuation rule of SARS-CoV-2 neutralizing antibody (NAb) in vivo is the key to prevent reinfection and recurrence of virus. Currently, the commonly used methods for detecting NAb include virus neutralization tests, pseudovirus neutralization assays, lateral flow immunochromatography and enzyme-linked immunosorbent assays. The detection of NAb not only can be used to evaluate the level of immunity after vaccination or infection but also can provide important theoretical support for virus reinfection, recurrence and vaccine iteration. In this research, the related technologies of SARS-CoV-2 NAb detection were reviewed, aiming to provide better research ideas for SARS-CoV-2 epidemic prevention and control.

Keywords: Detection method; Lateral flow; Neutralizing antibody; SARS-CoV-2.

Publication types

Review
Research Support, Non-U.S. Gov't

MeSH terms

Antibodies, Neutralizing*
Antibodies, Viral
COVID-19* / diagnosis
Humans
Reinfection
SARS-CoV-2

Substances

Antibodies, Neutralizing
Antibodies, Viral