Quantitation of Glutathione and Oxidized Glutathione Ratios from Biological Matrices Using LC-MS/MS

Thomas P Mathews

doi:10.1007/978-1-0716-3247-5_11

Quantitation of Glutathione and Oxidized Glutathione Ratios from Biological Matrices Using LC-MS/MS

Methods Mol Biol. 2023:2675:133-148. doi: 10.1007/978-1-0716-3247-5_11.

Author

Thomas P Mathews¹

Affiliation

¹ Children's Medical Center Research Institute, University of Texas Southwestern Medical Center, Dallas, TX, USA. thomas.mathews@UTSouthwestern.edu.

PMID: 37258761
DOI: 10.1007/978-1-0716-3247-5_11

Abstract

Oxidation of glutathione (GSH) to its disulfide dimer (GSSG) is the major mechanism by which cells balance reactive oxygen species (ROS) and mitigate oxidative stress. Thus, measuring the ratio of GSH/GSSG is an ideal way to assess oxidative stress within a cell. Quantitative mass spectrometry offers an ideal method to measure the GSH/GSSG ratio and can be applied to a variety of biological matrices and disease models. The following chapter details the design, optimization, and execution of a liquid chromatography-tandem mass spectrometry (LC-MS/MS) assay to measure the GSH/GSSG ratio.

Keywords: Glutathione; Liquid chromatography; Mass spectrometry; Oxidative stress; Oxidized glutathione; Quantitation; Reactive oxygen species.

MeSH terms

Chromatography, Liquid / methods
Glutathione Disulfide / analysis
Glutathione* / metabolism
Oxidation-Reduction
Oxidative Stress
Tandem Mass Spectrometry* / methods

Substances

Glutathione Disulfide
Glutathione