Role of non-coding RNAs against salinity stress in Oryza species: Strategies and challenges in analyzing miRNAs, tRFs and circRNAs

Int J Biol Macromol. 2023 Jul 1;242(Pt 3):125172. doi: 10.1016/j.ijbiomac.2023.125172. Epub 2023 Jun 1.

Abstract

Salinity is an imbalanced concentration of mineral salts in the soil or water that causes yield loss in salt-sensitive crops. Rice plant is vulnerable to soil salinity stress at seedling and reproductive stages. Different non-coding RNAs (ncRNAs) post-transcriptionally regulate different sets of genes during different developmental stages under varying salinity tolerance levels. While microRNAs (miRNAs) are well known small endogenous ncRNAs, tRNA-derived RNA fragments (tRFs) are an emerging class of small ncRNAs derived from tRNA genes with a demonstrated regulatory role, like miRNAs, in humans but unexplored in plants. Circular RNA (circRNA), another ncRNA produced by back-splicing events, acts as target mimics by preventing miRNAs from binding with their target mRNAs, thereby reducing the miRNA's action upon its target. Same may hold true between circRNAs and tRFs. Hence, the work done on these ncRNAs was reviewed and no reports were found for circRNAs and tRFs under salinity stress in rice, either at seedling or reproductive stages. Even the reports on miRNAs are restricted to seedling stage only, in spite of severe effects on rice crop production due to salt stress during reproductive stage. Moreover, this review sheds light on strategies to predict and analyze these ncRNAs in an effective manner.

Keywords: Abiotic stress; Non-coding RNAs; Salinity; circRNAs; miRNAs; tRFs.

Publication types

  • Review

MeSH terms

  • Humans
  • MicroRNAs* / genetics
  • MicroRNAs* / metabolism
  • Oryza* / genetics
  • Oryza* / metabolism
  • RNA, Circular / genetics
  • RNA, Circular / metabolism
  • RNA, Long Noncoding* / metabolism
  • RNA, Transfer / metabolism
  • RNA, Untranslated / genetics
  • Salt Tolerance
  • Seedlings / genetics
  • Seedlings / metabolism

Substances

  • MicroRNAs
  • RNA, Circular
  • RNA, Untranslated
  • RNA, Transfer
  • RNA, Long Noncoding