A Sensitive Spectrophotometric Assay of Pyruvate Dehydrogenase Activity

Anal Biochem. 1986 Jun;155(2):400-4. doi: 10.1016/0003-2697(86)90454-9.

Abstract

The recently reported highly sensitive method for assay of acetyl-CoA:arylamine N-acetyltransferase (EC 2.3.1.5) [H. H. Andres, A. J. Klein, S. M. Szabo, and W. W. Weber (1985) 145, 367-375] has been adapted for determination of pyruvate dehydrogenase activity. This method provides an improvement in sensitivity over extant spectrophotometric methods and circumvents limitations of assays using radioactive pyruvate. In addition, the assay is simple and inexpensive and can be readily adapted for measurement of enzyme activity in crude tissue extracts or homogenates.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Acetyl Coenzyme A / analysis
  • Animals
  • Arylamine N-Acetyltransferase / metabolism
  • Brain / enzymology
  • Columbidae
  • Liver / enzymology
  • Muscles / enzymology
  • Myocardium / enzymology
  • Pyruvate Dehydrogenase Complex / metabolism*
  • Rats
  • Spectrophotometry
  • Tissue Extracts / analysis

Substances

  • Pyruvate Dehydrogenase Complex
  • Tissue Extracts
  • Acetyl Coenzyme A
  • Arylamine N-Acetyltransferase